文章摘要
应用微卫星标记分析东兰乌鸡的遗传多样性
Analysis of Genetic Diversity of Guangxi blackone chickenUsing Microsatellite Markers
投稿时间:2014-10-31  修订日期:2014-12-17
DOI:
中文关键词: 东兰乌鸡  微卫星  遗传多样性  聚类分析
英文关键词: Blackone chicken  Microsatellite  GeneticSdiversity  cluster analysis
基金项目:
作者单位E-mail
莫国东 广西畜牧研究所 mgd9527@163.com 
黄英飞 广西畜牧研究所  
廖玉英 广西畜牧研究所  
韦凤英 广西畜牧研究所  
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中文摘要:
      【目的】探讨东兰乌鸡的遗传多样性。分析广西乌鸡品系间及其与其他鸡种间的亲缘关系,以期能够对广西乌鸡鸡种的遗传分化有更好地了解,为制订更有效的乌鸡种质资源保护与利用措施提供理论依据。【方法】采用微卫星标记方法分析广西地方品种东兰乌鸡的遗传多样性。通过计算等位基因、有效等位基因、杂合度、多态信息含量和遗传距离,评估东兰乌鸡品种内(丝羽、片羽)的遗传变异情况,并根据遗传距离与其他广西品种进行聚类分析。【结果】在18个微卫星标记中共检测到98个等位基因,每个位点的等位基因数在2-12个,平均等位基因数为5.44;平均多态性信息含量和杂合度分别为0.5382、0.5963;遗传距离和聚类分析结果与地方品种的进化、地理分布和选育历史基本一致。【结论】东兰乌鸡保种群具有丰富的遗传多样性,遗传资源在保种场得到妥善的保存。
英文摘要:
      【Objectiv】To?discuss?the?genetic?diversity?of?Guangxi?blackone?chicken, understand?the? genetic?differentiation?of?blackone?chicken?better?and?Provide?a?theoretical?basis?for?the?development?of?more?effective?protection?by?analyzing?the?genetic?relationship?between?blackone?chicken?and?the?other?Guangxi?chicken?breeds. 【Method】The genetic polymorphisms of microsatellite loci in Guangxi blackone chicken were analysed, allele, effective allele, heterozygosity (H), polymorphism information content (PIC) and genetic distance were calculated to the genetic variation in Guangxi blackone chicken (Silky feather and Contour feather). The dendrogram of blackone chicken and other Guangxi breeds was obtained based on genetic distance.【Result】The results showed that the 98 alleles were identified and the number of alleles varied from 2 to 12. And the mean number of alleles was 5.44n per locus. The mean PIC and H were 0.5382 and 0.5963 respectively. The genetic distance and UPGM were consistent with breeding history, geographical distribution of Guangxi breeds.【Conclusion】Guangxi blackone chicken preserved population have retained substantial biodiversity. The genetic resources are properly preserved in the conservation farms.
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