文章摘要
爬山虎与抗菌药联合对含fosA3大肠杆菌的抑菌作用
Study on the Effect of Parthenocissus tricuspidata Water Extractions Combined with Antibiotics Resistance Gene fosA3 in E.coli
投稿时间:2014-10-30  修订日期:2014-11-28
DOI:
中文关键词: 爬山虎  抗菌药  联合  含fosA3大肠杆菌  微量棋盘稀释法  FICI
英文关键词: Parthenocissus tricuspidata  Antibacterial agents  Combined induction  E.coli containingthe gene fosA3  Micrdilution checkerboard techniques  Fractional inhibitory concentration index
基金项目:国家自然科学基金(31460675) ;广西自然基金青年(2012GXNSFBA053052);广西自然基金面上(2013GXNSFAA019070);广西大学兽医一级学科博士启动(2012GXNSFBA053052);南宁市科技攻关项目(20141295)
作者单位E-mail
吴永继 广西大学 动物科学技术学院 wyjalisa@163.com 
刘增援 广西大学 动物科学技术学院  
王鹏霞 广西大学 动物科学技术学院  
孙燕杰 广西大学 动物科学技术学院  
宋剑武 广西大学 动物科学技术学院  
司红彬 广西大学 动物科学技术学院 zhuli_2009@163.com 
摘要点击次数: 1260
全文下载次数: 0
中文摘要:
      【目的】探讨爬山虎水煮液与抗菌药联合使用对含fosA3基因(Fosfomycin Resistance Gene)大肠杆菌的抑菌作用,为爬山虎的综合开发利用奠定基础。【方法】采用二倍微量稀释法检测爬山虎水煮液与抗菌药的最小抑菌浓度(MIC),然后用微量棋盘稀释法测定爬山虎水煮液与抗菌药联合作用后的部分抑菌浓度指数(FICI)。【结果】爬山虎水煮液的MIC值为0.5 g/ml,以0.5 g/ml爬山虎水煮液与头孢曲松钠、磷霉素、粘杆菌素、痢菌净、磺胺间甲氧嘧啶、阿米卡星、头孢他啶、头孢噻呋钠、氟苯尼考、头孢噻肟等10种抗菌药联合使用后的FICI值分别为0.563、1.000、0.313、0.750、1.000、1.000、0.258、0.531、0.188、0.750。【结论】爬山虎水煮液与抗菌药联合使用能增强对含fosA3大肠杆菌的体外抑菌作用,并呈现协同和相加作用。
英文摘要:
      Abstract:【Objective】This research was aimed to explore the antibacterial activity of bacterial which containing the gene fosA3 induced by water extractions of the Parthenocissus tricuspidata combined with antibacterial agents,providing reference for the comprehensive development and utilization of Parthenocissus tricuspidata.【Method】The minimal inhibiton concertration(MIC) of water extractions of Parthenocissus tricuspidata and antibacterial agents were tested by twice microdilution?method. Fractional inhibitory concentration indexes(FICI) of water extractions of Parthenocissus tricuspidata combined with antibacterial agents were determined by microdilution checkerboard techniques.【Result】The MIC of water extractions of Parthenocissus tricuspidata was 0.5 g/ml. The MIC of bacterial which containing the gene fosA3 was decreased obviously after inducing by 0.5g/ml of water extracts of Parthenocissus tricuspidata combined with 10 antibacterial agents, such as Ceftriaxone Sodium, Fosfomycin, Coly-mycin, Mequindox, Sulfamonomethoxine, Amikacin, Ceftazidime, Ceftiofur Sodium, Florfenicol, Cefotaxime Sodium. Fractional inhibitory concentration indexes(FICI) respectively were 0.563、1.000、0.313、0.750、1.000、1.000、0.258、0.531、0.188、0.750.【Conclusion】The antibacterial activities of antibacterial agents against bacterial which containing the gene fosA3 were significantly enforced by water extracts of Parthenocissus tricuspidata,showing synergism and additivity between water extracts of Parthenocissus tricuspidata and antibacterial agents.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=08EE5A5866ACE4403845AD45DCDBE8B1DA2D3551F2C5BEB1EF86575A826CF925EA412809BA643F571935CF177FC55F46F2291D41E0F612CF33271D064B852FFE538C6B83CD7F27249DBC008369B45160B18DDAE543C803C2846F620B375260713A966C0F5D5868D15D78B0C1D1496A6FEA14F518939A9652C3D6E3BC27649E7F&pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=522D78981551143623AB31CD59B20209&yid=&aid=&vid=&iid=&sid=&eid=&fileno=201410300000001&flag=2&is_more=0">